Pepsin Solution (Antigen Retrieval) (ab64201)
Overview
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Product name
Pepsin Solution (Antigen Retrieval) -
Tested applications
Suitable for: IHC-Pmore details -
General notes
Pepsin for protease-induced antigen retrieval (PIER) during IHC procedures (ab64201). Ready-to-use formulation. Tris buffer pH 2.0
Staining Protocol:
- Incubate tissue section for 10 minutes at 37°C.
- Rinse and proceed with immunostaining.
Pepsin is a commonly used digestive enzyme for immunohistochemical procedures.
Other products for IHC
Other enzymes for antigen retrieval include: Proteinase K Antigen Retrieval Solution ab64220 and Trypsin Antigen Retrieval Kit ab259256.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Properties
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Form
Liquid -
Storage instructions
Store at +4°C. -
Storage buffer
pH: 1.5
Preservative: 0.1% Sodium azide
Constituents: Tris buffer, 0.25% Pepsin -
Concentration information loading... -
Reagent notes
Pepsin is a commonly used digestive enzyme for immunohistochemical procedures. -
Research areas
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Function
Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. -
Sequence similarities
Belongs to the peptidase A1 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- PEPA_HUMAN
- Pepsin A
- Pepsinogen A5
see all -
Cellular localization
Secreted.
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Pepsin Solution (ab64201)ab64201 Pepsin Solution used with Laminin on Human Kidney