Recombinant human RIP protein (Active) (ab271732)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human RIP protein (Active)
See all RIP proteins and peptides -
Biological activity
Assay conditions: RIPK1 reactions was done in 25 μl containing 40 mM Tris, pH 7.4, 20 mM MgCl2, 0.1 % BSA, 3 mM DTT, 0.2 mg/ml MBP and 20 μM ATP at 30°C for 45 mins. The amount of ATP transferred to the substrate was measured using ADP-Glo reagents. -
Purity
>= 90 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MQPDMSLNVI KMKSSDFLES AELDSGGFGK VSLCFHRTQG LMIMKTVYKG PNCIEHNEAL LEEAKMMNRL RHSRVVKLLG VIIEEGKYSL VMEYMEKGNL MHVLKAEMST PLSVKGRIIL EIIEGMCYLH GKGVIHKDLK PENILVDNDF HIKIADLGLA SFKMWSKLNN EEHNELREVD GTAKKNGGTL YYMAPEHLND VNAKPTEKSD VYSFAVVLWA IFANKEPYEN AICEQQLIMC IKSGNRPDVD DITEYCPREI ISLMKLCWEA NPEARPTFPG IEEKFRPFYL SQLEESVEED VKSLKKEYSN ENAVVKRMQS LQLDCVA -
Predicted molecular weight
63 kDa -
Amino acids
1 to 327 -
Additional sequence information
N-terminal GST-Th tag.
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Preparation and Storage
- Cell death protein RIP
- FLJ39204
- OTTHUMP00000039163
Images
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SDS-PAGE - Recombinant human RIP protein (Active) (ab271732)
SDS-PAGE analysis of 2 µg ab271732.
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Functional Studies - Recombinant human RIP protein (Active) (ab271732)Specific activity of ab271732.