Recombinant human BRAF (mutated V600D) protein (ab204217)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
-
Product name
Recombinant human BRAF (mutated V600D) protein
See all BRAF proteins and peptides -
Biological activity
The specific activity of ab204217 was determined to be 2,350 nmol/min/mg.
-
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
DLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSS SSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDV AVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQ WCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSN NIFLHEDLTVKIGDFGLATDKSRWSGSHQFEQLSGSILWMAPEVIRMQDK NPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSK VRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASE PSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH -
Predicted molecular weight
69 kDa including tags -
Amino acids
381 to 766 -
Modifications
mutated V600D -
Tags
GST tag N-Terminus -
Additional sequence information
NM_004333.
-
Preparation and Storage
- FLJ95109
- 94 kDa B raf protein
- B raf
Images
-
SDS-PAGE - Recombinant human BRAF (mutated V600D) protein (ab204217)
The purity of ab204217 was determined to be >80% by densitometry.
Approximate MWt: 69 kDa. -
Functional Studies - Recombinant human BRAF (mutated V600D) protein (ab204217)Kinase assay showing the specific activity of ab204217 as 2,350 nmol/min/mg.
