Recombinant human BRAF (mutated V600E) protein (Active) (ab204154)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human BRAF (mutated V600E) protein (Active)
See all BRAF proteins and peptides -
Biological activity
The specific activity of ab204154 was determined to be 110 nmol/min/mg.
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Purity
> 70 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
LQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGS GSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILL FMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMD YLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATEKSRWSGSHQFEQLSG SILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQI IFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIE LLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPV H -
Predicted molecular weight
63 kDa including tags -
Amino acids
416 to 766 -
Modifications
mutated V600E -
Tags
GST tag N-Terminus -
Additional sequence information
NM_004333.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human BRAF (mutated V600E) protein (Active) (ab204154)The specific activity of BRAF (ab204154) was determined to be 1104 nmol/min/mg as per activity assay protocol and was equivalent to 98 nmol/min/mg as per radiometric assay
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SDS-PAGE - Recombinant human BRAF (mutated V600E) protein (Active) (ab204154)SDS PAGE analysis of ab204154 -
SDS-PAGE - Recombinant human BRAF (mutated V600E) protein (Active) (ab204154)SDS PAGE analysis of ab204154 -
Functional Studies - Recombinant human BRAF (mutated V600E) protein (Active) (ab204154)The specific activity of ab204154 was determined to be 110 nmol/min/mg.
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SDS-PAGE - Recombinant human BRAF (mutated V600E) protein (Active) (ab204154)SDS-PAGE analysis of ab204154 which was determined to be >90% pure by densitometry.
