Recombinant human BRAF (deleted L485 + N486 + V487 + T488 + A489, mutated P490Y) protein (ab204193)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human BRAF (deleted L485 + N486 + V487 + T488 + A489, mutated P490Y) protein
See all BRAF proteins and peptides -
Biological activity
The specific activity of ab204193 was determined to be 1500 nmol/min/mg.
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Purity
> 70 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
DLIRDQGFRG DGGSTTGLSA TPPASLPGSL TNVKALQKSP GPQRERKSSS SSEDRNRMKT LGRRDSSDDW EIPDGQITVG QRIGSGSFGT VYKGKWHGDV AVKMY TPQQLQAFKN EVGVLRKTRH VNILLFMGYS TKPQLAIVTQ WCEGSSLYHH LHIIETKFEM IKLIDIARQT AQGMDYLHAK SIIHRDLKSN NIFLHEDLTV KIGDFGLATV KSRWSGSHQF EQLSGSILWM APEVIRMQDK NPYSFQSDVY AFGIVLYELM TGQLPYSNIN NRDQIIFMVG RGYLSPDLSK VRSNCPKAMK RLMAECLKKK RDERPLFPQI LASIELLARS LPKIHRSASE PSLNRAGFQT EDFSLYACAS PKTPIQAGGY GAFPVH -
Predicted molecular weight
69 kDa including tags -
Amino acids
381 to 766 -
Modifications
deleted L485 + N486 + V487 + T488 + A489, mutated P490Y -
Tags
GST tag N-Terminus -
Additional sequence information
NM_004333. exon 9-18.
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Preparation and Storage
- FLJ95109
- 94 kDa B raf protein
- B raf
Images
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SDS-PAGE - Recombinant human BRAF (deleted L485 + N486 + V487 + T488 + A489, mutated P490Y) protein (ab204193)
SDS-PAGE analysis of ab204193.
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Functional Studies - Recombinant human BRAF (deleted L485 + N486 + V487 + T488 + A489, mutated P490Y) protein (ab204193)Kinase Assay demonstrating specific activity of ab204193.
