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Neuroscience Neurotransmission Intracellular Signaling Kinases

Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)

Price and availability

341 740 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2606Y] to PKA alpha/beta/gamma (catalytic subunit) (phospho T197)
  • Suitable for: WB, IHC-P, Dot blot
  • Reacts with: Human

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Overview

  • Product name

    Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y]
    See all PKA alpha/beta/gamma (catalytic subunit) primary antibodies
  • Description

    Rabbit monoclonal [EP2606Y] to PKA alpha/beta/gamma (catalytic subunit) (phospho T197)
  • Host species

    Rabbit
  • Specificity

    Detects PKA alpha/beta/gamma (catalytic subunit) phosphorylated on threonine 197. Moreover, the antibody will cross-react with PRKX/Y (pT203).
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human PKA alpha/beta/gamma (catalytic subunit) aa 150-250 (phospho T197). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa cell lysate treated with calyculin A.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP2606Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Kinases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKC
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKA
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • Other
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Western blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    Western blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    All lanes : Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991) at 1/1000 dilution

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) 10% FBS all the time. Whole cell lysates
    Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) serum starve for 24h then 20% FBS 20h. Whole cell lysates
    Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) serum starve for 24h then 20% FBS 20h.Whole cell lysates.Then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa


    Exposure time: 1 second


    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Dot Blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    Dot Blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)

    Dot blot analysis of various peptides labelling PKA gamma (catalytic subunit) (phospho T197) with ab75991 at a dilution of 1/1000. ab97051, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/100000).

    Lane 1 - PKA alpha/beta/gamma (catalytic subunit) (pT197) phospho peptide.

    Lane 2 - PKA alpha/beta/gamma (catalytic subunit) non-phospho peptide.

    Lane 3 - PRKX/Y (pT203) phospho peptide.

    Lane 4 - PRKX/Y non-phospho peptide.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    Western blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    All lanes : Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991) at 1/5000 dilution

    Lane 1 : HeLa whole cell lysate - treated with Calyculin A with PKA alpha/beta/gamma (catalytic subunit) (pT197) phospho peptide
    Lane 2 : HeLa whole cell lysate - treated with Calyculin A with PKA alpha/beta/gamma (catalytic subunit) non-phospho peptide
    Lane 3 : HeLa whole cell lysate - treated with Calyculin A with PRKX/Y (pT203) phospho peptide
    Lane 4 : HeLa whole cell lysate - treated with Calyculin A with PRKX/Y non- phospho peptide

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    Western blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    All lanes : Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991) at 1/5000 dilution

    Lane 1 : HeLa cell lysates, untreated
    Lane 2 : HeLa cell lysates treated with calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat-anti rabbit IgG at 1/2000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)

    ab75991, at a 1/100 dilution, staining PKA gamma (catalytic subunit) in formalin fixed, paraffin embedded human testis tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)
    Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991)

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  • Product image

    Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] - BSA and Azide free (ab227848)

    Applications: Dot, IHC-P, WB

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