Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FANCA/FAA antibody [EPR23667-61] - BSA and Azide free (ab272392)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23667-61] to FANCA/FAA - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-FANCA/FAA antibody [EPR23667-61] - BSA and Azide free
    See all FANCA/FAA primary antibodies

  • Description

    Rabbit monoclonal [EPR23667-61] to FANCA/FAA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse thymus, testis and embryo lysates; Rat testis lysate; NIH/3T3 whole cell lysate. IHC-P: Mouse testis tissue. IP: MEF whole cell lysate.

  • General notes

    ab272392 is the carrier-free version of ab242151. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCA/FAA antibody [EPR23667-61] - BSA and Azide free (ab272392)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCA/FAA antibody [EPR23667-61] - BSA and Azide free (ab272392)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling FANCA/FAA with ab242151 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse testis (PMID: 10754110, PMID: 16397136). The section was incubated with ab242151 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242151).

  • Immunoprecipitation - Anti-FANCA/FAA antibody [EPR23667-61] - BSA and Azide free (ab272392)
    Immunoprecipitation - Anti-FANCA/FAA antibody [EPR23667-61] - BSA and Azide free (ab272392)

    FANCA/FAA was immunoprecipitated from 0.35 mg MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate with ab242151 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab242151 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate 10 ug

    Lane 2: ab242151 IP in MEF whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab242151 in MEF whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This blot was developed using a higher sensitivity ECL substrate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242151).

  • Anti-FANCA/FAA antibody [EPR23667-61] - BSA and Azide free (ab272392)
    Anti-FANCA/FAA antibody [EPR23667-61] - BSA and Azide free (ab272392)

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