Anti-Cytochrome C antibody [EP1326-80-5-4] - BSA and Azide free (ab247454)
![Anti-Cytochrome C antibody [EP1326-80-5-4] - BSA and Azide free (ab247454)](https://www.abcam.com/ps/products/247/ab247454/Images/ab247454-405545-anti-cytochrome-c-antibody-ep1326-80-5-4-bsa-and-azide-free-western-blot.jpg "Anti-Cytochrome C antibody [EP1326-80-5-4] - BSA and Azide free (ab247454)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1326-80-5-4] to Cytochrome C - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Cytochrome C antibody [EP1326-80-5-4] - BSA and Azide free
See all Cytochrome C primary antibodies -
Description
Rabbit monoclonal [EP1326-80-5-4] to Cytochrome C - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247454 is the carrier-free version of ab76237. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247454 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP1326-80-5-4 -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Images
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Western blot - Anti-Cytochrome C antibody [EP1326-80-5-4] - BSA and Azide free (ab247454)Anti-Cytochrome C antibody [EP1326-80-5-4] (ab76237) at 1/500 dilution + HeLa cell lysate at 10 µg
Secondary
goat anti-rabbit HRP at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?This data was developed using ab76237, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EP1326-80-5-4] - BSA and Azide free (ab247454)](https://www.abcam.com/ps/products/247/ab247454/Images/ab247454-2-anti-cytochrome-c-antibody-ep1326-80-5-4-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EP1326-80-5-4] - BSA and Azide free (ab247454)This data was developed using ab76237, the same antibody clone in a different buffer formulation.ab76237, at a 1/100 dilution, staining human Cytochrome C in heart muscle by Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EP1326-80-5-4] - BSA and Azide free (ab247454)](https://www.abcam.com/ps/products/247/ab247454/Images/ab247454-1-anti-cytochrome-c-antibody-ep1326-80-5-4-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EP1326-80-5-4] - BSA and Azide free (ab247454)This data was developed using ab76237, the same antibody clone in a different buffer formulation.ab76237, at a 1/100 dilution, staining human Cytochrome C in kidney by Immunohistochemistry Formalin/PFA-fixed paraffin-embedded tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. -
![Anti-Cytochrome C antibody [EP1326-80-5-4] - BSA and Azide free (ab247454)](https://www.abcam.com/ps/products/247/ab247454/Images/ab247454-4-benefits-of-recombinant-antibodies.png)
Anti-Cytochrome C antibody [EP1326-80-5-4] - BSA and Azide free (ab247454)
