Human CXADR Antibody Pair - BSA and Azide free (ab253659)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human CXADR Antibody Pair - BSA and Azide free -
Assay type
ELISA set -
Range
15.6 pg/ml - 1000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
- Human CXADR Antibody Pair is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human CXADR in sandwich ELISAs and many other pair-based applications.
- The pair can be used in variety of assays and platforms including but not limited to:
- - Sandwich ELISA
- - FRET/TR-FRET/HTR
- - Meso Scale Discovery® (MSD®)
- - Luminex® and bead-based assays
- - AlphaLISA®/AlphaScreen®
- - DELFIA® immunoassays
- - Simoa® and Single Molecule Counting (SMC™) immunoassays
- - Multiplex
- Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
- - Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
- - Concentration of ~1 mg/ml as measured by the protein A280 method.
- Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
- We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
- Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
- Please note:
- The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
- The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
- We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
- Antibody properties:
- Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg
- Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg
- Concentration: ~1 mg/ml
- Storage buffer: 100% PBS
- Form: Liquid
- Isotype: IgG
- Recombinant monoclonal antibodies offer several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
- For more information see here.
- Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
- Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
- Luminex is a trademark of Luminex Corporation, registered in the US and other countries.
- AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
- Simoa is a registered trademark of Quanterix, Inc.
- SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components Identifier 10 x 96 tests Human CXADR Capture Antibody (unconjugated) — Human CXADR Detector Antibody (unconjugated) — -
Function
Component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN. -
Tissue specificity
Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
The Ig-like C2-type 1 domain probably mediates homodimerization and interaction with JAML.
The PDZ-binding motif mediates interaction with MPDZ and BAIAP1. -
Post-translational
modificationsN-glycosylated.
Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane. -
Cellular localization
Secreted and Cell membrane. Cell junction > tight junction. Cell junction > adherens junction. Basolateral cell membrane. In epithelial cells localizes to the apical junction complex composced of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface. - Information by UniProt
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Alternative names
- 46 kD coxsackievirus and adenovirus receptor CAR protein
- 46 kD coxsackievirus and adenovirus receptor protein
- CAR
see all -
Database links
- Entrez Gene: 1525 Human
- Omim: 602621 Human
- SwissProt: P78310 Human
- Unigene: 634837 Human
Images
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Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253659)To learn more about the advantages of recombinant antibodies see here.