Human Apolipoprotein B Antibody Pair - BSA and Azide free (ab256598)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human Apolipoprotein B Antibody Pair - BSA and Azide free
See all Apolipoprotein B kits -
Assay type
ELISA set -
Range
14 ng/ml - 900 ng/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human Apolipoprotein B. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab190806), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human Apolipoprotein B Capture Antibody (unconjugated) 1 x 100µg Human Apolipoprotein B Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. -
Involvement in disease
Hypobetalipoproteinemia, familial, 1
Familial ligand-defective apolipoprotein B-100
Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia. -
Sequence similarities
Contains 1 vitellogenin domain. -
Post-translational
modificationsPalmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. -
Cellular localization
Cytoplasm. Secreted. - Information by UniProt
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Alternative names
- Apo B
- Apo B 100
- Apo B-100
see all -
Database links
- Entrez Gene: 338 Human
- Omim: 107730 Human
- SwissProt: P04114 Human
- Unigene: 120759 Human
Images
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Sandwich ELISA - Human Apolipoprotein B Antibody Pair - BSA and Azide free (ab256598)To learn more about the advantages of recombinant antibodies see here.
