Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATP5G1/G2/G3 antibody [EPR13908] - BSA and Azide free (ab250158)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13908] to ATP5G1/G2/G3 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ATP5G1/G2/G3 antibody [EPR13908] - BSA and Azide free
    See all ATP5G1/G2/G3 primary antibodies

  • Description

    Rabbit monoclonal [EPR13908] to ATP5G1/G2/G3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250158 is the carrier-free version of ab180149. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250158 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] - BSA and Azide free (ab250158)
    Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] - BSA and Azide free (ab250158)
    All lanes : Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149) at 1/2000 dilution (purified)

    Lane 1 : mouse heart lysate
    Lane 2 : rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 14 kDa
    Observed band size: 8 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab180149, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] - BSA and Azide free (ab250158)
    Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] - BSA and Azide free (ab250158)
    Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149) at 1/2000 dilution (purified) + human fetal heart tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 14 kDa
    Observed band size: 8 kDa why is the actual band size different from the predicted?



    This data was developed using ab180149, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] - BSA and Azide free (ab250158)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] - BSA and Azide free (ab250158)
    This data was developed using ab180149, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded human hepatocellular carcinoma with purified ab180149 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] - BSA and Azide free (ab250158)
    Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] - BSA and Azide free (ab250158)
    All lanes : Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149) at 1/2000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Human fetal heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 14 kDa



    This data was developed using ab180149, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] - BSA and Azide free (ab250158)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] - BSA and Azide free (ab250158)
    This data was developed using ab180149, the same antibody clone in a different buffer formulation.Immuohistochemical analysis of Paraffin-embedded Human liver tissue labeling ATP5G1 with unpurified ab180149 at 1/100 dilution, followed by prediluted Goat anti rabbit IgG (HRP). Counter stained with Hematoxylin.
  • Immunoprecipitation - Anti-ATP5G1/G2/G3 antibody [EPR13908] - BSA and Azide free (ab250158)
    Immunoprecipitation - Anti-ATP5G1/G2/G3 antibody [EPR13908] - BSA and Azide free (ab250158)
    This data was developed using ab180149, the same antibody clone in a different buffer formulation.Western blot analysis of fetal heart lysate immunoprecipitated with unpurified ab180149 at 1/30 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
  • Anti-ATP5G1/G2/G3 antibody [EPR13908] - BSA and Azide free (ab250158)
    Anti-ATP5G1/G2/G3 antibody [EPR13908] - BSA and Azide free (ab250158)

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