Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (ab251357)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPRR18188] to REG3A + REG3G - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free
    See all REG3A + REG3G primary antibodies

  • Description

    Rabbit monoclonal [EPRR18188] to REG3A + REG3G - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251357 is the carrier-free version of ab202057. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251357 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPRR18188

  • Isotype

    IgG

Images

  • Western blot - Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (ab251357)
    Western blot - Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (ab251357)
    All lanes : Anti-REG3A + REG3G antibody [EPRR18188] (ab202057) at 1/1000 dilution

    Lane 1 : Human small intestine tissue lysate
    Lane 2 : Human fetal liver tissue lysate
    Lane 3 : Human fetal brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 16 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab202057, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literatures PMID: 12890698 & 22943287 for REG3A and PMID: 22252863 & 21254165 for REG3G.

    Please note both Human fetal liver and brain represent negative controls for REG3A and REG3G.

  • Western blot - Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (ab251357)
    Western blot - Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (ab251357)
    All lanes : Anti-REG3A + REG3G antibody [EPRR18188] (ab202057) at 1/10000 dilution

    Lane 1 : REG3A recombinant protein fragment
    Lane 2 : REG3G recombinant protein fragment

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 16 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This data was developed using ab202057, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (ab251357)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (ab251357)
    This data was developed using ab202057, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling REG3G + REG3A with ab202057 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on Human pancreas tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (ab251357)
    Immunocytochemistry - Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (ab251357)
    This data was developed using ab202057, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized BxPC-3 (Human pancreas adenocarcinoma cells) cells labeling REG3G + REG3A with ab202057 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on BxPC-3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:
    -ve control 1: ab202057 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
  • Immunocytochemistry - Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (ab251357)
    Immunocytochemistry - Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (ab251357)
    This data was developed using ab202057, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized HT-29 (Human colorectal adenocarcinoma cells) cells labeling REG3G + REG3A with ab202057 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HT-29 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:
    -ve control 1: ab202057 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
  • Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (ab251357)
    Anti-REG3A + REG3G antibody [EPRR18188] - BSA and Azide free (ab251357)

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