Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling Cytokeratin 10 with ab183317 at 1/100 dilution (0.95 µg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 1 (pH 6.0) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human skin, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183317 for 10 mins at room temperature.

This image was generated using ab183317, the same clone, but with a different buffer formulation.

Immunohistochemistry (Frozen) analysis of rat skin tissue section labeling Cytokeratin 10 with purified ab183317 at 1/50 (1.9 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This image was generated using ab183317, the same clone, but with a different buffer formulation.

Immunohistochemistry (Frozen) analysis of mouse skin tissue section labeling Cytokeratin 10 with purified ab183317 at 1/50 (1.9 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This image was generated using ab183317, the same clone, but with a different buffer formulation.

Immunohistochemical analysis of Human skin labeling Cytokeratin 10 using ab183317 at a 1/100 dilution

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183317).

Immunohistochemical analysis of Human skin labeling Cytokeratin 10 using ab183317 at a 1/100 dilution

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183317).

Immunohistochemical analysis of Human skin squamous cell carcinoma labeling Cytokeratin 10 using ab183317 at a 1/100 dilution

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183317).

Immunohistochemical analysis of human cervical squamous cell carcinoma labeling Cytokeratin 10 using ab183317 at a 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183317).