Anti-CRISPR-Cas9 antibody [EPR19619-92] - BSA and Azide free (ab223548)
![Anti-CRISPR-Cas9 antibody [EPR19619-92] - BSA and Azide free (ab223548)](https://www.abcam.com/ps/products/223/ab223548/Images/ab223548-367596-anti-crispr-cas9-antibody-epr19619-92-immunohistochemistry.jpg "Anti-CRISPR-Cas9 antibody [EPR19619-92] - BSA and Azide free (ab223548)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19619-92] to CRISPR-Cas9 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Streptococcus pyogenes
Overview
-
Product name
Anti-CRISPR-Cas9 antibody [EPR19619-92] - BSA and Azide free
See all CRISPR-Cas9 primary antibodies -
Description
Rabbit monoclonal [EPR19619-92] to CRISPR-Cas9 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Streptococcus pyogenes, Streptococcus thermophilus -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HEK-293 transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-tag, whole cell lysate; HEK-293 transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, N–terminal aa1-800) with GFP-tag, whole cell lysate.
-
General notes
Ab223548 is the carrier-free version of ab210752. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab223548 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19619-92 -
Isotype
IgG
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-92] - BSA and Azide free (ab223548)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded 293T cells transfected with Streptococcus Thermophilus CRISPR-Cas9 (pcDNA3.1(+)-Myc-His) with ab210752 at 1/50 dilution. Goat Anti-Rabbit IgG H&L (HRP), ab97051 at 1/500 dillution was used as the secondary antibody. Counterstained with Hematoxylin. Secondary only negative control also shown. Heat mediated antigen retrieval using Tris/EDTA Buffer, pH 9.0 was performed.
Positive staining on the 293T cells transfected with Streptococcus Thermophilus Cas9.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210752).
-
![Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-92] - BSA and Azide free (ab223548)](https://www.abcam.com/ps/products/223/ab223548/Images/ab223548-318790-141.jpg)
Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-92] - BSA and Azide free (ab223548)All lanes : Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752) at 1/5000 dilution
Lane 1 : Untransfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate (control)
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-tag
Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, N–terminal aa1-800) with GFP-tag
Lane 4 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, C–terminal aa801-1409) with GFP-tag
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 164 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
The immunogen is located at the N-terminus.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210752).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-92] - BSA and Azide free (ab223548)](https://www.abcam.com/ps/products/223/ab223548/Images/ab223548-367597-anti-crispr-cas9-antibody-epr19619-92-immunohistochemistry-293t.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-92] - BSA and Azide free (ab223548)Immunohistochemical analysis of formalin-fixed, paraffin-embedded 293T cells transfected blank pcDNA3.1(+)-Myc-His vector with ab210752 at 1/50 dilution. Goat Anti-Rabbit IgG H&L (HRP), ab97051 at 1/500 dillution was used as the secondary antibody. Counterstained with Hematoxylin. Secondary only negative control also shown. Heat mediated antigen retrieval using Tris/EDTA Buffer, pH 9.0 was performed.
Negative on the 293T cells transfected blank pcDNA3.1(+)-Myc-His vector.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210752).
-
![Anti-CRISPR-Cas9 antibody [EPR19619-92] - BSA and Azide free (ab223548)](https://www.abcam.com/ps/products/223/ab223548/Images/ab223548-4-benefits-of-recombinant-antibodies.png)
Anti-CRISPR-Cas9 antibody [EPR19619-92] - BSA and Azide free (ab223548)
