Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Amyloid Fibril antibody [mOC78] - BSA and Azide free (ab251428)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [mOC78] to Amyloid Fibril - BSA and Azide free
  • Suitable for: Dot blot, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Amyloid Fibril antibody [mOC78] - BSA and Azide free
    See all Amyloid Fibril primary antibodies

  • Description

    Rabbit monoclonal [mOC78] to Amyloid Fibril - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Other Immunogen Type corresponding to Human Amyloid Fibril. (Amyloid beta 1-42 fibrils).
    Database link: P05067

  • General notes

    ab251428 is the carrier-free version of ab205341 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251428 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed as part of a collaboration between Abcam and Professor Charles Glabe, UC Irvine.

    ab251428 (mOC78) recognizes a conformation-dependent and aggregation-specific discontinuous epitope of beta Amyloid that maps to segments 8-11 (SGYE), 18-24  (VFFAEDV) and 26-32 (SNKGAII) (Hatami et al 2014). It  also recognizes a-synuclein and islet amyloid polypeptide (IAPP) fibrils but not monomers on dot blots.  It recognizes a broad range of bands from monomer to high molecular weight with samples of Aß40 and Aß42 on western blots (Hatami et al 2014).  Immunoreactivity on western blots is not changed by boiling the membrane. mOC78 stains plaques and a unique population of intraneuronal and intranuclear amyloid and also stains the center of nascent neuritic plaques where it colocalizes with neuronal chromatin (Pensalfani et al 2014). 

    For further information on the immunogen, please refer to Hatami et al. 2014 and Kayed et al. 2007.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amyloid Fibril antibody [mOC78] - BSA and Azide free (ab251428)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amyloid Fibril antibody [mOC78] - BSA and Azide free (ab251428)
    This data was developed using ab205341, the same antibody clone in a different buffer formulation.IHC image of Amyloid Fibrillin staining in Human Brain Alzheimer formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab205341, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
  • Dot Blot - Anti-Amyloid Fibril antibody [mOC78] - BSA and Azide free (ab251428)
    Dot Blot - Anti-Amyloid Fibril antibody [mOC78] - BSA and Azide free (ab251428)
    This data was developed using ab205341, the same antibody clone in a different buffer formulation.Dot blot analysis of beta Amyloid labeled with ab205341 at 1/6000 dilution. Lane 1: beta Amyloid (Aβ) 1-40; Lane 2: beta Amyloid (Aβ) 1-42. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/30000 was used as secondary antibody. Blocking/Dilution buffer: 5% NFDM/TBST. Exposure time: 30 seconds. Note: Antibody reactivity was assessed using a dot blot, which is a non-quantitative method that maintains the native conformation of beta Amyloid. beta Amyloid 1-40 and 1-42 peptides underwent the following aggregation conditions before being spotted onto a nitrocellulose membrane and detected using ab205341: Monomers: 0.3 mg of beta Amyloid peptide was dissolved in 30 µl 100 mM NaOH and incubated at room temperature for 10 minutes. It was then diluted with 970 µl of 1% SDS and boiled for five minutes. Oligomers: 0.3 mg of beta Amyloid peptide was dissolved in 30 µl 100 mM NaOH and incubated at room temperature for 10 minutes. It was then diluted with 970 µl of 10 mM phosphate buffer pH 7.4 containing 0.02% sodium azide and incubated at room temperature for four days. Fibrils: 0.3 mg of beta Amyloid peptide was dissolved in 1 ml 50% hexafluoroisopropanol (HFIP) with 0.02% sodium azide. It was then stirred constantly for nine days; the first seven with a cap on and the final two with the cap removed to allow evaporation of the HFIP. Fibrils were then sedimented at 20,000 rpm in a microcentrifuge for 20 minutes and resuspended in 1 ml of PBS + 0.02% sodium azide.
  • Dot Blot - Anti-Amyloid Fibril antibody [mOC78] - BSA and Azide free (ab251428)
    Dot Blot - Anti-Amyloid Fibril antibody [mOC78] - BSA and Azide free (ab251428)
    This data was developed using ab205341, the same antibody clone in a different buffer formulation.Negative control (secondary ab only) Dot blot analysis of beta Amyloid. Lane 1: beta Amyloid (Aβ) 1-40; Lane 2: beta Amyloid (Aβ) 1-42. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/30000 was used as secondary antibody. Blocking/Dilution buffer: 5% NFDM/TBST. Exposure time: 30 seconds.
  • Anti-Amyloid Fibril antibody [mOC78] - BSA and Azide free (ab251428)
    Anti-Amyloid Fibril antibody [mOC78] - BSA and Azide free (ab251428)

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