Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Chondroitinase ABC I antibody [HL2010] - BSA and Azide free (ab264484)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [HL2010] to Chondroitinase ABC I - BSA and Azide free
  • Suitable for: ELISA, WB
  • Reacts with: Recombinant fragment

Overview

  • Product name

    Anti-Chondroitinase ABC I antibody [HL2010] - BSA and Azide free
    See all Chondroitinase ABC I primary antibodies

  • Description

    Mouse monoclonal [HL2010] to Chondroitinase ABC I - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: ELISA, WBmore details

  • Species reactivity

    Reacts with: Recombinant fragment

  • Immunogen

    Recombinant fragment within Chondroitinase ABC I aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Proteus vulgaris.
    Database link: P59807

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Proteus vulgaris Chondroitinase ABC recombinant Protein (aa25-1021).

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab264484 is the carrier-free version of ab256485. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    HL2010

  • Isotype

    IgG1

Images

  • Western blot - Anti-Chondroitinase ABC I antibody [HL2010] - BSA and Azide free (ab264484)
    Western blot - Anti-Chondroitinase ABC I antibody [HL2010] - BSA and Azide free (ab264484)
    Anti-Chondroitinase ABC I antibody [HL2010] (ab256485) at 0.092 µg/ml + Proteus vulgaris Chondroitinase ABC recombinant Protein (aa25-1021), 20 ng

    Secondary
    Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This image was produced using the same antibody clone but in a different formulation ab256485, PBS, sodium azide, glycerol and BSA.

  • ELISA - Anti-Chondroitinase ABC I antibody [HL2010] - BSA and Azide free (ab264484)
    ELISA - Anti-Chondroitinase ABC I antibody [HL2010] - BSA and Azide free (ab264484)

    ELISA - Anti-Chondroitinase ABC I antibody [HL2010] (ab256485).

    Antigen: Chondroitinase ABC at 1000 ng/ml.

    ab256485 used at 20-5000 ng/mL.

    Secondary is an Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) used at 1/1000 dilution.

    This image was produced using the same antibody clone but in a different formulation ab256485, PBS, sodium azide, glycerol and BSA.

  • Anti-Chondroitinase ABC I antibody [HL2010] - BSA and Azide free (ab264484)
    Anti-Chondroitinase ABC I antibody [HL2010] - BSA and Azide free (ab264484)

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