Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-APPL antibody [EPR13569] (ab180140)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13569] to APPL
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-APPL antibody [EPR13569]
    See all APPL primary antibodies

  • Description

    Rabbit monoclonal [EPR13569] to APPL

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human APPL aa 400-500 (internal sequence). The exact sequence is proprietary.
    Database link: Q9UKG1

  • Positive control

    • WB: HeLa, MCF7 and 293 lysates. IHC-P: Human colonic carcinoma and Human pancreas tissue. ICC: MCF7 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-APPL antibody [EPR13569] (ab180140)
    Western blot - Anti-APPL antibody [EPR13569] (ab180140)
    All lanes : Anti-APPL antibody [EPR13569] (ab180140) at 1/10000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : APPL knockout HeLa cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 80 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?



    Lanes 1- 4: Merged signal (red and green). Green - ab180140 observed at 90 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab180140 was shown to react with Anti-APPL in wild-type HeLa cells in western blot. The band observed in knockout cell line ab265187 (knockout cell lysate ab257836) lane below 90kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and APPL1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab180140 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-APPL antibody [EPR13569] (ab180140)
    Western blot - Anti-APPL antibody [EPR13569] (ab180140)
    All lanes : Anti-APPL antibody [EPR13569] (ab180140) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 80 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-APPL antibody [EPR13569] (ab180140)
    Immunocytochemistry/ Immunofluorescence - Anti-APPL antibody [EPR13569] (ab180140)

    Immunofluorescent analysis of 4% paraformaldehyde fixed MCF7 cells labeling APPL with ab180140 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(Dylight 555) Conterstained with Dapi blue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [EPR13569] (ab180140)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [EPR13569] (ab180140)

    Immunohistochemical analysis of paraffin-embedded Human pancreas carcinoma tissue labeling APPL with ab180140 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [EPR13569] (ab180140)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [EPR13569] (ab180140)

    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling APPL with ab180140 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-APPL antibody [EPR13569] (ab180140)
    Anti-APPL antibody [EPR13569] (ab180140)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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