Anti-APPL antibody - N-terminal (ab226185)
Key features and details
- Rabbit polyclonal to APPL - N-terminal
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-APPL antibody - N-terminal
See all APPL primary antibodies -
Description
Rabbit polyclonal to APPL - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human APPL aa 1-50 (N terminal). The exact sequence is proprietary.
Database link: Q9UKG1-1 -
Positive control
- IP: HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226185 was affinity purified using an epitope specific to APPL immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-APPL antibody - N-terminal (ab226185)
APPL was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226185 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226185 at 0.4 µg/ml.
Lane 1: ab226185 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
