Protein Creatinine Ratio Assay Kit (ab272539)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Sample type: Urine
Overview
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Product name
Protein Creatinine Ratio Assay Kit
See all Creatinine kits -
Detection method
Colorimetric -
Sample type
Urine -
Assay type
Quantitative -
Product overview
Protein Creatinine Ratio Assay Kit (ab272539) is a simple and convenient test for proteinuria. Other methods such as 24 hour urine test or timed urine test require strict adherence to sample collection protocol. Since the protein concentration is normalized to creatinine secretion, the urine sample can be taken at anytime and no diet or liquid restrictions are necessary for sample collection.
Sensitive and accurate: Use 20 μL samples. Linear detection range in 96-well plate: 1 - 20 mg/dL Protein and 1 – 150 mg/dL Creatinine.
Fast and convenient: No sample pre-treatment is needed. Simple 10- minute "add-incubate-read" procedure.
High-throughput adaptable: The procedure can be readily automated for processing thousands of samples per day.
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Tested applications
Suitable for: Functional Studiesmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests CR Reagent A 1 x 6ml CR Reagent B 1 x 6ml PR Reagent 1 x 24ml Standard 1 x 1ml -
Research areas
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Relevance
Creatinine, or creatine anhydride, is a breakdown product of creatine phosphate in muscle. The loss of water molecule from creatine results in the formation of creatinine. Creatinine is transferred to the kidneys by blood plasma, whereupon it is eliminated from the body by glomerular filtration and partial tubular excretion. Creatinine is usually produced and excreted at a fairly constant rate, and blood creatinine is used to determine glomerular filtration rate (GFR), a measure of kidney function.
Images
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Protein Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Example Protein standard curve.
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Creatinine Standard CurveTypical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Eample Creatinine standard curve.