Recombinant Human CENPB protein (His tag) (ab275796)
Key features and details
- Expression system: Escherichia coli
- Purity: > 97% SDS-PAGE
- Endotoxin level:
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
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Product name
Recombinant Human CENPB protein (His tag)
See all CENPB proteins and peptides -
Purity
> 97 % SDS-PAGE. -
Endotoxin level
Expression system
Escherichia coliAccession
Protein length
Protein fragmentAnimal free
NoNature
Recombinant-
Species
Human -
Sequence
GPKRRQLTF REKSRIIQEV EENPDLRKGE IARRFNIPPS TLSTILKNKR AILASERKYG VASTCRKTNK LSPYDKLEGL LIAWFQQIRA AGLPVKGIIL KEKALRIAEE LGMDDFTASN GWLDRFRRRH GVVSCS -
Predicted molecular weight
19 kDa -
Molecular weight information
19kDa by SDS-PAGE reducing conditions. -
Amino acids
2 to 136 -
Tags
His tag N-Terminus
Specifications
Our Abpromise guarantee covers the use of ab275796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8
Constituents: 0.24% Tris, 0.87% Sodium chloride, 0.01% Sarkosyl, 5% Trehalose -
ReconstitutionReconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
General Info
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Alternative names
- CENP B
- CENP-B
- CENPB
see all -
Function
Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes. -
Sequence similarities
Contains 1 HTH CENPB-type DNA-binding domain.
Contains 1 HTH psq-type DNA-binding domain. -
Cellular localization
Nucleus. Chromosome > centromere. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human CENPB protein (His tag) (ab275796)
SDS-PAGE analysis of ab275796.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab275796 has not yet been referenced specifically in any publications.
Preparation and Storage
- CENP B
- CENP-B
- CENPB
Images
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SDS-PAGE - Recombinant Human CENPB protein (His tag) (ab275796)
SDS-PAGE analysis of ab275796.