Anti-Bid antibody (ab272880)
Key features and details
- Rabbit polyclonal to Bid
- Suitable for: WB, ICC/IF, IHC-P, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Bid antibody
See all Bid primary antibodies -
Description
Rabbit polyclonal to Bid -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Full length protein corresponding to Human Bid.
Database link: P55957 -
Positive control
- WB: HEK-293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, Rat2, RAW 264.7 and C2C12 whole cell lysate. IHC-P: U87 xenograft tissue. ICC/IF: A431 and HeLa cells. IP: Jurkat cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Bid antibody (ab272880)All lanes : Anti-Bid antibody (ab272880) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : H1299 (human non-small cell lung carcinoma cell line) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 6 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Lane 7 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.7.5 % SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bid antibody (ab272880)Paraffin-embedded U87 xenograft tissue stained for Bid using ab272880 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-Bid antibody (ab272880)4% PFA-fixed A431 (human epidermoid carcinoma cell line) cells stained for Bid using ab272880 at 1/1000 dilution in ICC/IF.
Red: alpha Tubulin, a cytoskeleton marker.
Blue: Hoechst 33342 staining.
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Immunoprecipitation - Anti-Bid antibody (ab272880)Immunoprecipitation of Bid protein from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extracts using 5 μg of ab272880 or ab137752.
Western blot analysis was performed using ab272880 diluted at 1:500, followed by anti-Rabbit IgG.Lane 1: Input.
Lane 2: Control IgG.
Lane 3: ab272880 IP.
Lane 4: ab137752 IP.
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Immunocytochemistry/ Immunofluorescence - Anti-Bid antibody (ab272880)2% paraformaldehyde/culture medium fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Bid using ab272880 at 1/1000 dilution in ICC/IF.
Blue: Hoechst 33343.
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Western blot - Anti-Bid antibody (ab272880)Anti-Bid antibody (ab272880) at 1/1000 dilution + Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg12% SDS-PAGE
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Western blot - Anti-Bid antibody (ab272880)All lanes : Anti-Bid antibody (ab272880) at 1/500 dilution
Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : C2C12 (mouse myoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.12% SDS-PAGE
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Western blot - Anti-Bid antibody (ab272880)All lanes : Anti-Bid antibody (ab272880) at 1/1000 dilution
Lane 1 : Non transfected HEK293T whole cell lysate
Lane 2 : Bid shRNA transfected HEK293T whole cell lysate
Lysates/proteins at 60 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG12% SDS-PAGE