Lysosome Isolation Kit (ab234047)
Key features and details
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Lysosome Isolation Kit -
Sample type
Tissue, Adherent cells, Suspension cells -
Product overview
Lysosome Isolation Kit (ab234047) provides a procedure for isolating an enriched or purified lysosomal fraction from animal tissues and cultured cells by differential centrifugation followed by density gradient centrifugation.
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Notes
Lysosomes are membrane-bound cell organelles found in most animal cells (except red blood cells). Lysosomes are spherical vesicles containing hydrolytic enzymes capable of breaking down different kinds of biomolecules, including proteins, nucleic acids, carbohydrates, lipids, and cellular debris. They are known to contain over 50 different enzymes, which are active under acidic conditions. Thus, lysosomes act as the waste disposal system of the cells by digesting unwanted materials in the cytoplasm.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 50 tests Lysosome Enrichment Buffer 1 x 100ml Lysosome Gradient 1 x 85ml Lysosome Isolation Buffer 1 x 25ml Protease Inhibitor Cocktail 1 x 1ml
Images
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Location of lysosome band in Discontinuous Density Gradient following ultracentrifugation.
Tentative Lysosome band location in gradient density.
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Acid Phosphatase (biomarker enzyme for lysosomes) specific activity (mU/mg protein) increases with each purification stepData is normalized by the amount of protein used per well.
