Anti-CD53 antibody [MRC OX-44] - BSA and Azide free (ab244561)
![Anti-CD53 antibody [MRC OX-44] - BSA and Azide free (ab244561)](https://www.abcam.com/ps/products/244/ab244561/Images/ab244561-1-ab22383Flow1.jpg "Anti-CD53 antibody [MRC OX-44] - BSA and Azide free (ab244561)")
Key features and details
- Mouse monoclonal [MRC OX-44] to CD53 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-CD53 antibody [MRC OX-44] - BSA and Azide free
See all CD53 primary antibodies -
Description
Mouse monoclonal [MRC OX-44] to CD53 - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Rat
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Immunogen
Tissue, cells or virus corresponding to Rat CD53. T lymphocytes from a mixed lymphocyte reaction between purified rat T helper cells and irradiated semiallogeneic rat spleen
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Positive control
- Flow Cyt: Lewis rat splenocytes.
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General notes
ab244561 is the PBS only version of ab22383.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
MRC OX-44 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-CD53 antibody [MRC OX-44] - BSA and Azide free (ab244561)
This data was generating using the same clone in a different formulation (ab22383).
Lewis rat splenocytes stained with ab22383 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab22383) or mouse IgG1κ Isotype (ab170190) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
