Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD53 antibody [EPR4342(2)] (ab134094)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4342(2)] to CD53
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD53 antibody [EPR4342(2)]
    See all CD53 primary antibodies

  • Description

    Rabbit monoclonal [EPR4342(2)] to CD53

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues on the C terminus in Human CD53 (UniProt P19397).

  • Positive control

    • Raji, PBMC, and Jurkat whole cell lysate (ab7899); Human tonsil tissue

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR4342(2)

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD53 antibody [EPR4342(2)] (ab134094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD53 antibody [EPR4342(2)] (ab134094)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD53 with ab134094 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-CD53 antibody [EPR4342(2)] (ab134094)
    Western blot - Anti-CD53 antibody [EPR4342(2)] (ab134094)
    All lanes : Anti-CD53 antibody [EPR4342(2)] (ab134094) at 1/1000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : PBMC cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 24 kDa

  • Flow Cytometry - Anti-CD53 antibody [EPR4342(2)] (ab134094)
    Flow Cytometry - Anti-CD53 antibody [EPR4342(2)] (ab134094)

    Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling CD53 with unpurified ab134094 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Anti-CD53 antibody [EPR4342(2)] (ab134094)
    Anti-CD53 antibody [EPR4342(2)] (ab134094)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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