Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CLCA1 antibody [EPR12254] - BSA and Azide free (ab249816)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12254] to CLCA1 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CLCA1 antibody [EPR12254] - BSA and Azide free
    See all CLCA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR12254] to CLCA1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Recombinant fragment corresponding to Human CLCA1.
    Database link: A8K7I4

  • General notes

    Ab249816 is the carrier-free version of ab174319. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249816 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CLCA1 antibody [EPR12254] - BSA and Azide free (ab249816)
    Western blot - Anti-CLCA1 antibody [EPR12254] - BSA and Azide free (ab249816)
    All lanes : Anti-CLCA1 antibody [EPR12254] (ab174319) at 1/10000 dilution

    Lane 1 : Human colon cancer lysate
    Lane 2 : Human small intestine lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 100 kDa



    This data was developed using ab174319, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254] - BSA and Azide free (ab249816)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254] - BSA and Azide free (ab249816)
    This data was developed using ab174319, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CLCA1 with ab174319 at 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-CLCA1 antibody [EPR12254] - BSA and Azide free (ab249816)
    Immunoprecipitation - Anti-CLCA1 antibody [EPR12254] - BSA and Azide free (ab249816)
    This data was developed using ab174319, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from Human colon cancer lysate using ab174319 at 1/10 dilution.
  • Anti-CLCA1 antibody [EPR12254] - BSA and Azide free (ab249816)
    Anti-CLCA1 antibody [EPR12254] - BSA and Azide free (ab249816)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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