Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Myf5 antibody [EPR4899] (ab125078)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4899] to Myf5
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Myf5 antibody [EPR4899]
    See all Myf5 primary antibodies

  • Description

    Rabbit monoclonal [EPR4899] to Myf5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Myf5 aa 200 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • WB: Human skeletal muscle, rat muscle, mouse muscle and rat heart tissue lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Myf5 antibody [EPR4899] (ab125078)
    Western blot - Anti-Myf5 antibody [EPR4899] (ab125078)
    Anti-Myf5 antibody [EPR4899] (ab125078) at 1/50000 dilution (purified) + Human skeletal muscle tissue lysate at 10 µg

    Secondary
    HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 28 kDa
    Observed band size: 39 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST
  • Western blot - Anti-Myf5 antibody [EPR4899] (ab125078)
    Western blot - Anti-Myf5 antibody [EPR4899] (ab125078)
    All lanes : Anti-Myf5 antibody [EPR4899] (ab125078) at 1/50000 dilution (purified)

    Lane 1 : Rat muscle tissue lysate
    Lane 2 : Mouse muscle tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 28 kDa
    Observed band size: 39 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST
  • Immunoprecipitation - Anti-Myf5 antibody [EPR4899] (ab125078)
    Immunoprecipitation - Anti-Myf5 antibody [EPR4899] (ab125078)

    ab125078 (Purified) at 1/20 dilution (20 µg/ml) immunoprecipitating Myf5 in Human skeletal muscle lysate.

    Lane 1 (input): Human skeletal muscle
    Lane 2 (+): 125078 & Human skeletal muscle lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab125078 in human skeletal muscle lysate
    For western blotting, ab125078 at 1/1000 and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM /TBST.

  • Western blot - Anti-Myf5 antibody [EPR4899] (ab125078)
    Western blot - Anti-Myf5 antibody [EPR4899] (ab125078)
    All lanes : Anti-Myf5 antibody [EPR4899] (ab125078) at 1/10000 dilution (unpurified)

    Lane 1 : Human skeletal muscle tissue lysate
    Lane 2 : Rat muscle tissue lysate
    Lane 3 : Mouse muscle tissue lysate
    Lane 4 : Rat heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 28 kDa

  • Anti-Myf5 antibody [EPR4899] (ab125078)
    Anti-Myf5 antibody [EPR4899] (ab125078)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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