Filariasis ELISA kit (ab247194)
Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (qualitative)
- Reacts with: Human
Overview
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Product name
Filariasis ELISA kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% sample 24 0.435nM 2.07% sample 24 0.334nM 3.13% sample 24 0.143nM 3.97% Inter-assay Sample n Mean SD CV% sample 10 11.85nM 1.81% sample 10 9.45nM 2.67% sample 10 11.91nM 2.6% -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Sandwich (qualitative) -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Filariasis ELISA Kit (ab247194) is intended for the qualitative determination of specific antibodies against filariae in human serum or plasma (citrate or heparin).
The qualitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique.
Microplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product.
The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA microwell plate reader.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover Foil 1 unit Filariasis Coated Microplate 1 unit Filariasis Cut-off Control 1 x 3ml Filariasis Negative Control 1 x 2ml Filariasis Positive Control 1 x 2ml Protein A/G HRP conjugate 1 x 20ml Sample Diluent 1 x 100ml Stop Solution red cap 1 x 15ml TMB Substrate Solution Yellow cap 1 x 15ml
Images
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Serologic ELISA assay principleSpecific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
