Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DAP12 antibody [EPR24244-119] - BSA and Azide free (ab280577)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24244-119] to DAP12 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-DAP12 antibody [EPR24244-119] - BSA and Azide free
    See all DAP12 primary antibodies

  • Description

    Rabbit monoclonal [EPR24244-119] to DAP12 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW 264.7, N9 lysates. IP: RAW 264.7 cells

  • General notes

    ab280577 is the carrier-free version of ab280568. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280577 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24244-119

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-DAP12 antibody [EPR24244-119] - BSA and Azide free (ab280577)
    Western blot - Anti-DAP12 antibody [EPR24244-119] - BSA and Azide free (ab280577)
    All lanes : Anti-DAP12 antibody [EPR24244-119] (ab280568) at 1/1000 dilution

    Lane 1 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 2 : N9 (mouse microglia) whole cell lysate
    Lane 3 : M1 (mouse myeloid leukemia myeloblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 12 kDa



    This data was developed using ab280568, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: M1 (PMID: 11034384).

    Exposure time: 3 minutes

     

  • Immunoprecipitation - Anti-DAP12 antibody [EPR24244-119] - BSA and Azide free (ab280577)
    Immunoprecipitation - Anti-DAP12 antibody [EPR24244-119] - BSA and Azide free (ab280577)

    This data was developed using ab280568, the same antibody clone in a different buffer formulation.

    DAP12 was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 ug with ab280568 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280568 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 ug

    Lane 2: ab280568 IP in RAW 264.7 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab280568 in RAW 264.7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 24 seconds

     

  • Anti-DAP12 antibody [EPR24244-119] - BSA and Azide free (ab280577)
    Anti-DAP12 antibody [EPR24244-119] - BSA and Azide free (ab280577)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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