Anti-CITED1 antibody (ab87978)
Key features and details
- Mouse monoclonal to CITED1
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
Overview
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Product name
Anti-CITED1 antibody -
Description
Mouse monoclonal to CITED1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISA, IHC-Pmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Cow, Pig -
Immunogen
Recombinant fragment: SMQLQKLNSQ YQGMAAATPG QPGEAGPLQN WDFGAQAGGA ESLSPSAGAQ SPAIIDSDPV DEEVLMSLVV ELGLDRANEL PELWLGQNEF DFTADFPSSC , corresponding to amino acids 94-194 of Human CITED1 with proprietary tag. (NP_004134)
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Positive control
- Purchase matching WB positive control:Recombinant Human CITED1 protein
- Human Testis and A-431 cell lysate. CITED1 transfected 293T cell lysate
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General notes
This product was changed from ascites to tissue culture supernatant on 12/3/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Anti-CITED1 antibody (ab87978) at 5 µg/ml + A-431 cell lysate at 50 µg
Predicted band size: 20 kDa
Observed band size: 20 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.This image was generated using the ascites version of the product.
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All lanes : Anti-CITED1 antibody (ab87978) at 1 µg/ml
Lane 1 : Transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 20 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?This image was generated using the ascites version of the product.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CITED1 antibody (ab87978)
Immunoperoxidase of monoclonal antibody ab87978 at 1.2 ug/ml to CITED1 on formalin-fixed paraffin-embedded Human testis.
This image was generated using the ascites version of the product.
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Capture ELISA using recombinant protein. Detection limit is approximately 0.03ng/ml as a capture antibody.
This image was generated using the ascites version of the product.
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Anti-CITED1 antibody (ab87978) at 5 µg/ml + Recombinant protein used as immunogen at 0.2 µg/ml
Predicted band size: 20 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?This image was generated using the ascites version of the product.