Anti-CrkL antibody (ab233234)
Key features and details
- Rabbit polyclonal to CrkL
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-CrkL antibody
See all CrkL primary antibodies -
Description
Rabbit polyclonal to CrkL -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human CrkL aa 1 to the C-terminus. N-terminal tags. Expressed in E. coli.
Database link: P46109 -
Positive control
- IHC-P: Human liver and prostate tissue. WB: Recombinant human CrkL protein. Pig brain tissue lysate. HeLa, HepG2 and K562 cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CrkL antibody (ab233234)All lanes : Anti-CrkL antibody (ab233234) at 3 µg/ml
Lane 1 : Pig brain tissue lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Secondary
All lanes : HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 34 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CrkL antibody (ab233234)Formalin-fixed, paraffin-embedded human liver tissue stained for CrkL with ab233234 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CrkL antibody (ab233234)Formalin-fixed, paraffin-embedded human prostate tissue stained for CrkL with ab233234 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Western blot - Anti-CrkL antibody (ab233234)Anti-CrkL antibody (ab233234) at 3 µg/ml + Recombinant human CrkL protein
Predicted band size: 34 kDa
