Anti-CrkL antibody (ab151791)
Key features and details
- Rabbit polyclonal to CrkL
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CrkL antibody
See all CrkL primary antibodies -
Description
Rabbit polyclonal to CrkL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Xenopus laevis
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-282 of Human CrkL.
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Positive control
- 293T, A431, H1299, HeLa S3, HepG2, Molt-4, Raji and Mouse brain whole cell lysates; HeLa cells; Human SW480 xenograft.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CrkL antibody (ab151791)All lanes : Anti-CrkL antibody (ab151791) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLaS3 whole cell lysate
Lane 5 : HepG2 whole cell lysate
Lane 6 : Molt-4 whole cell lysate
Lane 7 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 34 kDa
12% SDS PAGE -
Western blot - Anti-CrkL antibody (ab151791)Anti-CrkL antibody (ab151791) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 34 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-CrkL antibody (ab151791)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling CrkL with ab151791 at 1/200 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CrkL antibody (ab151791)Immunohistochemical analysis of paraffin-embedded SW480 xenograft labeling CrkL with ab151791 at 1/500 dilution.
