Anti-CrkL (phospho Y251) antibody (ab215751)
Key features and details
- Rabbit polyclonal to CrkL (phospho Y251)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CrkL (phospho Y251) antibody
See all CrkL primary antibodies -
Description
Rabbit polyclonal to CrkL (phospho Y251) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human CrkL (phospho Y251) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P46109 -
Positive control
- K562 cell lysate stimulated with pervanadate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CrkL (phospho Y251) antibody (ab215751)Lanes 1-2 : Rabbit anti-CrkL (C-terminus)
Lanes 3-4 : Anti-CrkL (phospho Y251) antibody (ab215751) at 1/1000 dilution
Lanes 1 & 3 : K562 cell lysate stimulated with pervanadate (1mM) for 30 min
Lanes 2 & 4 : K562 cell lysate stimulated with pervanadate (1mM) for 30 min treated with alkaline phosphatase
Predicted band size: 34 kDa
Observed band size: 38, 40 kDa why is the actual band size different from the predicted?
