Antibodies, Proteins, Kits and Reagents for Life Science

271 382 ₸ Product size

100 µl 271 382 ₸ 1 ml 2 010 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [CAL61] to GITR
Suitable for: WB, Flow Cyt, IHC-P
Reacts with: Mouse

Product name

Anti-GITR antibody [CAL61]
See all GITR primary antibodies
Description

Rabbit monoclonal [CAL61] to GITR
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
IHC-P	Mouse
WB	Mouse

See all applications and species data

Immunogen

Synthetic peptide within Mouse GITR aa 175-228. The exact sequence is proprietary.
Database link: O35714
Positive control
- IHC-P: Mouse lung and spleen tissue. WB: Mouse splenocytes treated with concanavalin A. Flow Cyt: Mouse splenocytes treated with concanavalin A.
General notes

This product was previously labeled as TNFRSF18.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein A purified
Purification notes

Purity >99%
Clonality

Monoclonal
Clone number

CAL61
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL61] (ab237725)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling GITR with ab237725 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the mouse lung is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL61] (ab237725)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling GITR with ab237725 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the mouse spleen is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-GITR antibody [CAL61] (ab237725)

All lanes : Anti-GITR antibody [CAL61] (ab237725) at 1/1000 dilution

Lane 1 : Untreated mouse splenocytes whole cell lysate
Lane 2 : Mouse splenocytes treated with 2.5µg/ml concanavalin A for 3 days whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 26 kDa

Exposure time: 3 minutes

33 kDa band represents GITR isoform B based on Uniprot and literature. (PMID: 10836847).

Blocking/Dilution buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-GITR antibody [CAL61] (ab237725)

Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse splenocytes treated with 2.5μg/ml concanavalin A for 3 days labeling GITR with ab237725 at 1/500 (right panel) compared with a Rabbit monoclonal IgG (ab172730) (left panel). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with anti-CD25 conjugated to BV421. Then fixed with 2% PFA followed by intracellularly stained with rabbit IgG (Left) or ab237725 (Right).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL61] (ab237725)

Formalin-fixed, paraffin-embedded mouse spleen tissue stained for GITR using ab237725 at 0.125 µg/ml dilution in immunohistochemical analysis.
Anti-GITR antibody [CAL61] (ab237725)

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