Antibodies, Proteins, Kits and Reagents for Life Science

Anti-GITR antibody [BLR068G] - BSA free (ab264419)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR068G] to GITR - BSA free
Suitable for: IP, WB, IHC-P
Reacts with: Human

Product name

Anti-GITR antibody [BLR068G] - BSA free
See all GITR primary antibodies
Description

Rabbit monoclonal [BLR068G] to GITR - BSA free
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human GITR aa 191-241 (C terminal). The exact sequence is proprietary. NP_004186.1.
Database link: Q9Y5U5
Positive control
- ICC: RPMI-8226 cells. IHC-P: Human tonsil tissue. WB: MJ, RPMI-8226 and C5/MJ whole cell lysates.
General notes

This product is sold under License from Bethyl Laboratories, Inc

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline
Concentration information loading...
Purification notes

Purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR068G
Isotype

IgG
Research areas

Western blot - Anti-GITR antibody [BLR068G] - BSA free (ab264419)

All lanes : Anti-GITR antibody [BLR068G] - BSA free (ab264419) at 1/1000 dilution

Lane 1 : MJ whole cell lysate at 15 µg
Lane 2 : Jurkat whole cell lysate at 50 µg
Lane 3 : RMPI-8226 whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Lane 5 : Raji whole cell lysate at 50 µg
Lane 6 : MOLT4 whole cell lysate at 50 µg
Lane 7 : Daudi whole cell lysate at 50 µg
Lane 8 : C5/MJ whole cell lysate at 15 µg

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG

Developed using the ECL technique.

Predicted band size: 26 kDa

Exposure time: 30 seconds

Lysates prepared using NETN buffer.
Immunocytochemistry - Anti-GITR antibody [BLR068G] - BSA free (ab264419)

Immocytochemistry analysis of formalin-fixed, paraffin embedded human RPMI-8226 cells labeling GITR with ab264419 at a 1/125 dilution. HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [BLR068G] - BSA free (ab264419)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling GITR with ab264419 at 1/200 dilution. Secondary: DyLight^® 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [BLR068G] - BSA free (ab264419)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling GITR with ab264419 at 1/125 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunoprecipitation - Anti-GITR antibody [BLR068G] - BSA free (ab264419)

GITR was immunoprecipitated from MJ whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264419 at 6 µl per reaction. Western blot was performed from the immunoprecipitates using ab264419 at 1/1000.

Lane 1: ab264419 IP in MJ whole cell lysate.
Lane 2: Control IgG IP in MJ whole cell lysate.

Detection: Chemiluminescence with an exposure time of 3 seconds.

Lysates prepared using NETN lysis buffer.
Anti-GITR antibody [BLR068G] - BSA free (ab264419)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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