Anti-GITR antibody [EPR20566] (ab223841)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20566] to GITR
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
-
Product name
Anti-GITR antibody [EPR20566]
See all GITR primary antibodies -
Description
Rabbit monoclonal [EPR20566] to GITR -
Host species
Rabbit -
Specificity
We found ab223841 worked well in WB with recombinant protein only. The glycosylation of the endogenous target resulted in different molecular masses on the blot. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HEK-293T transfected with human GITR, containing a Myc-GFP-tag, whole cell lysate. IHC-P: Human tonsil, colon and Hodgkin's lymphoma tissues. ICC/IF: HEK-293T cells transfected with GFP-tagged GITR expression vector.
-
General notes
This product was previously labelled as TNFRSF18
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20566 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling GITR with ab223841 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in sporadic cells (arrows) in human Hodgkin’s lymphoma (PMID: 25787078; PMID: 23785514). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells, transfected with GFP only or with GFP-tagged GITR expression vector, labeling GITR with ab223841 at 1/500 dilution followed by by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079) secondary antibody (white) at 1/500 dilution. Confocal image showing cytoplasmic staining in HEK-293T cells transfected with GFP-tagged GITR expression vector.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079) secondary antibody at 1/500 dilution.
-
All lanes : Anti-GITR antibody [EPR20566] (ab223841) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control) containing a Myc-GFP-tag whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with human GITR, containing a Myc-GFP-tag, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
-
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling GITR with ab223841 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in stromal cells (arrows) in human colon (PMID: 25787078; PMID: 23785514). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling GITR using ab223841 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in sporadic cells (arrows) in human tonsil (PMID: 25787078; PMID: 23785514). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-