Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CAL52] to GITR - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-GITR antibody [CAL52] - BSA and Azide free
    See all GITR primary antibodies

  • Description

    Rabbit monoclonal [CAL52] to GITR - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil tissue. ICC/IF: HEK-293T cells. IP: HEK-293T and Hut-78 whole cell lysate. Flow: Human peripheral blood mononuclear cells.

  • General notes

    ab251600 is the carrier-free version of ab237713 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251600 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as TNFRSF18

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling GITR with ab237713 at 1/4000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the human tonsil is observed. Counter stained with Hematoxylin. The section was incubated with ab237713 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab237713).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for TNFSF18 using ab237713 at 0.25 μg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab237713).

  • Immunocytochemistry/ Immunofluorescence - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)
    Immunocytochemistry/ Immunofluorescence - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)

    4% Paraformaldehyde-fixed 0.1% TritonX-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labeling GITR with ab237713 at 1/50 dilution followed by a AlexaFluor®594 Goat anti-Rabbit secondary (ab150080) at a 1/500 dilution (Green). The nuclear counterstain was DAPI (Blue). Confocal image showing Positive staining in HEK-293T cells transfected with a GFP-tagged GITR expression construct.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®594 Goat anti-Rabbit secondary (ab150080) at a 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab237713). 

  • Flow Cytometry - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)
    Flow Cytometry - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)

    Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% tween-20 permeabilized Human peripheral blood mononuclear cell (PBMC) treated with 10μg/ml PHA for 48h, labeling GITR with ab237713 at 1/500 dilution. The secondary antibody was a Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/500 dilution. Cells were surface stained with anti-CD25 conjugated to BV421. Then fixed with 2% PFA followed by intracellular staining rabbit IgG (Left) or ab237713 (Right). The isotype control used was a Rabbit monoclonal IgG (ab172730, Left).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab237713).

  • Immunoprecipitation - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)
    Immunoprecipitation - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)

    GITR was immunoprecipitated from 0.35 mg Hut-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate using ab237713 at 1/30 dilution. western blot was performed on the immunoprecipitate using ab237713 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

    Lane 1: Hut-78 whole cell lysate 10 μg (input)
    Lane 2: ab237713 IP in Hut-78 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237713 in Hut-78 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

    This blot was developed using a higher sensitivity ECL substrate.

    Dimerized GITR was also observed at 52kDa

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab237713).

  • Immunoprecipitation - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)
    Immunoprecipitation - Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)

    GITR was immunoprecipitated from 0.35 mg HEK-293T (Human embryonic kidney epithelial cell) transfected with GFP-tagged GITR overexpression vector whole cell lysate using ab237713 at 1/30 dilution. western blot was performed on the immunoprecipitate using ab237713 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

    Lane 1: Hut-78 whole cell lysate 10 μg (input)
    Lane 2: ab237713 IP in Hut-78 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237713 in 293T transfected with GFP-tagged GITR overexpression vector whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This blot was developed using a higher sensitivity ECL substrate.

    Dimerized GITR was also observed at 52kDa

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab237713).

  • Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)
    Anti-GITR antibody [CAL52] - BSA and Azide free (ab251600)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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