Rat CTGF ELISA Kit (ab275897)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.12 ng/ml
- Range: 0.16 ng/ml - 10 ng/ml
- Sample type: Cell culture media, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat CTGF ELISA Kit
See all CTGF kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2.9% Inter-assay Sample n Mean SD CV% Serum 4 8.1% -
Sample type
Serum, Cell culture media, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.12 ng/ml -
Range
0.16 ng/ml - 10 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 104 97% - 110% Cell culture media 96 88% - 100% EDTA Plasma 89 81% - 94% Cit plasma 105 98% - 110% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat CTGF ELISA kit (ab275897) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Rat CTGF protein in rat serum, plasma (EDTA), plasma citrate, cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat CTGF with 0.12 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Rat connective tissue growth factor (CTGF), also known as CCN2, and Insulin-like growth factor-binding protein 8, is a matricellular protein encoded by the gene CTGF. Along with CYR61 (CCN1) and NOV (CCN3), CTGF belongs to the CCN family of extracellular matrix-associated heparin binding proteins. The CCN proteins contain IGFBP, Von Willebrand type C repeats, and thrombospodin type 1 repeat domains. CTGF has multiple functions including promotion proliferation and differentiation of chondrocytes; mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells, and enhances fibroblast growth factor-induced DNA synthesis.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat CTGF Capture Antibody 1 x 600µl 10X Rat CTGF Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Plate Seals 1 unit Rat CTGF Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. -
Tissue specificity
Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested. -
Sequence similarities
Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain. -
Cellular localization
Secreted > extracellular space > extracellular matrix. Secreted. - Information by UniProt
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Alternative names
- CCN 2
- CCN family member 2
- CCN2
see all -
Database links
- Entrez Gene: 64032 Rat
- SwissProt: Q9R1E9 Rat
- Unigene: 17145 Rat
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of rat CTGF standard curve in Sample Diluent NS.The CTGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CTGF in rat serum, plasma (EDTA), and plasma (citrate).The concentrations of CTGF were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (EDTA) 5%, and plasma (citrate) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 199 ng/mL in serum, 140 ng/mL in plasma (EDTA) and 172 ng/mL in and plasma (citrate).
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Sandwich ELISA - Rat CTGF ELISA Kit (ab275897)To learn more about the advantages of recombinant antibodies see here.
