Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CRMP2 (phospho T514) antibody (ab85934)

Key features and details

  • Rabbit polyclonal to CRMP2 (phospho T514)
  • Suitable for: ICC/IF, WB, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-CRMP2 (phospho T514) antibody
    See all CRMP2 primary antibodies

  • Description

    Rabbit polyclonal to CRMP2 (phospho T514)

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Orangutan

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab99478)

  • Positive control

    • This antibody gave a positive signal in human, mouse and rat brain tissue lysates.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab85934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IP Use a concentration of 5 µg/ml.

Target

  • Function

    Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.

  • Post-translational
    modifications

    3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Collapsin response mediator protein 2 antibody
    • Collapsin response mediator protein antibody
    • Collapsin response mediator protein hCRMP 2 antibody
    • CRAM antibody
    • CRMP 2 antibody
    • CRMP-2 antibody
    • CRMP2 antibody
    • DHPRP 2 antibody
    • DHPRP2 antibody
    • Dihydropyrimidinase 2 antibody
    • Dihydropyrimidinase like 2 antibody
    • Dihydropyrimidinase like 2 long form antibody
    • Dihydropyrimidinase related protein 2 antibody
    • Dihydropyrimidinase-related protein 2 antibody
    • DPYL 2 antibody
    • DPYL2 antibody
    • DPYL2_HUMAN antibody
    • DPYSL 2 antibody
    • Dpysl2 antibody
    • DRP-2 antibody
    • DRP2 antibody
    • Musunc 33 antibody
    • Musunc33 antibody
    • N2A3 antibody
    • TOAD 64 antibody
    • TOAD64 antibody
    • ULIP 2 protein antibody
    • ULIP-2 antibody
    • Ulip2 antibody
    • Unc-33-like phosphoprotein 2 antibody
    see all

Images

  • Western blot - Anti-CRMP2 (phospho T514) antibody (ab85934)
    Western blot - Anti-CRMP2 (phospho T514) antibody (ab85934)
    All lanes : Anti-CRMP2 (phospho T514) antibody (ab85934) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466) with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Phosphorylated T 514)
    Lane 5 : Brain (Mouse) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Phosphorylated T 514)
    Lane 6 : Brain (Rat) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Phosphorylated T 514)
    Lane 7 : Human brain tissue lysate - total protein (ab29466) with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Non-Modified)
    Lane 8 : Brain (Mouse) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Non-Modified)
    Lane 9 : Brain (Rat) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Non-Modified)

    Lysates/proteins at 10 µg per lane.

    Blocking peptides at 1 µg/ml per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 62 kDa
    Observed band size: 62,70 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    The predicted molecular weight of is 62-kDa (SwissProt), however in mouse and rat brain tissue lysates, we observe a band at 70-kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • Immunocytochemistry/ Immunofluorescence - Anti-CRMP2 (phospho T514) antibody (ab85934)
    Immunocytochemistry/ Immunofluorescence - Anti-CRMP2 (phospho T514) antibody (ab85934)

    ICC/IF image of ab85934 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85934 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunoprecipitation - Anti-CRMP2 (phospho T514) antibody (ab85934)
    Immunoprecipitation - Anti-CRMP2 (phospho T514) antibody (ab85934)
    CRMP2 was immunoprecipitated using 0.5mg Mouse Brain tissue, 5µg of Rabbit polyclonal to CRMP2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab85934.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 70kDa; CRMP2

References (1)

Publishing research using ab85934? Please let us know so that we can cite the reference in this datasheet.

ab85934 has been referenced in 1 publication.

  • Akama K  et al. Proteomic identification of differentially expressed genes in neural stem cells and neurons differentiated from embryonic stem cells of cynomolgus monkey (Macaca fascicularis) in vitro. Biochim Biophys Acta 1814:265-76 (2011). WB ; Monkey . PubMed: 21047566

Images

  • Western blot - Anti-CRMP2 (phospho T514) antibody (ab85934)
    Western blot - Anti-CRMP2 (phospho T514) antibody (ab85934)
    All lanes : Anti-CRMP2 (phospho T514) antibody (ab85934) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466) with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Phosphorylated T 514)
    Lane 5 : Brain (Mouse) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Phosphorylated T 514)
    Lane 6 : Brain (Rat) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Phosphorylated T 514)
    Lane 7 : Human brain tissue lysate - total protein (ab29466) with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Non-Modified)
    Lane 8 : Brain (Mouse) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Non-Modified)
    Lane 9 : Brain (Rat) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Non-Modified)

    Lysates/proteins at 10 µg per lane.

    Blocking peptides at 1 µg/ml per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 62 kDa
    Observed band size: 62,70 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    The predicted molecular weight of is 62-kDa (SwissProt), however in mouse and rat brain tissue lysates, we observe a band at 70-kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • Immunocytochemistry/ Immunofluorescence - Anti-CRMP2 (phospho T514) antibody (ab85934)
    Immunocytochemistry/ Immunofluorescence - Anti-CRMP2 (phospho T514) antibody (ab85934)

    ICC/IF image of ab85934 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85934 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunoprecipitation - Anti-CRMP2 (phospho T514) antibody (ab85934)
    Immunoprecipitation - Anti-CRMP2 (phospho T514) antibody (ab85934)
    CRMP2 was immunoprecipitated using 0.5mg Mouse Brain tissue, 5µg of Rabbit polyclonal to CRMP2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab85934.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 70kDa; CRMP2

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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