Antibodies, Proteins, Kits and Reagents for Life Science

526 012 ₸ Product size

100 µg 526 012 ₸ 1 mg 4 690 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3023] to Epsin 1 - BSA and Azide free
Suitable for: ICC, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free
See all Epsin 1 primary antibodies
Description

Rabbit monoclonal [EPR3023] to Epsin 1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: ICC, WBmore details
Unsuitable for: IHC-P
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab247415 is the carrier-free version of ab75879. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247415 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3023
Isotype

IgG
Research areas

Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)

Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/5000 dilution (purified) + rat brain at 20 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 58 kDa
Observed band size: 94 kDa
why is the actual band size different from the predicted?

This data was developed using ab75879, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)

All lanes : Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/1000 dilution (purified)

Lane 1 : 293T cell lysate
Lane 2 : K562 cell lysate
Lane 3 : U-87 MG cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 58 kDa
Observed band size: 94 kDa why is the actual band size different from the predicted?

This data was developed using ab75879, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)

Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/5000 dilution (purified) + mouse brain tissue lysate at 20 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 58 kDa
Observed band size: 94 kDa why is the actual band size different from the predicted?

This data was developed using ab75879, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Immunocytochemistry - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)

This data was developed using ab75879, the same antibody clone in a different buffer formulation.Immunofluorescence staining of HeLa cells with purified ab75879 at a working dilution of 1/50, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab75879 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
Immunocytochemistry - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)

This data was developed using ab75879, the same antibody clone in a different buffer formulation.Unpurified ab75879, at a 1/100 dilution, staining Epsin 1 in SH-SY5Y cells by Immunofluorescence.
Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)

All lanes : Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/2000 dilution (unpurified)

Lane 1 : SH-SY5Y cell lysate
Lane 2 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit-HRP at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 58 kDa

This data was developed using ab75879, the same antibody clone in a different buffer formulation.
Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com