Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3023] to Epsin 1 - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free
    See all Epsin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3023] to Epsin 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WBmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247415 is the carrier-free version of ab75879. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247415 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
    Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
    Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/5000 dilution (purified) + rat brain at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 58 kDa
    Observed band size: 94 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab75879, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
    Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
    All lanes : Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/1000 dilution (purified)

    Lane 1 : 293T cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : U-87 MG cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size: 58 kDa
    Observed band size: 94 kDa why is the actual band size different from the predicted?



    This data was developed using ab75879, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
    Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
    Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/5000 dilution (purified) + mouse brain tissue lysate at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size: 58 kDa
    Observed band size: 94 kDa why is the actual band size different from the predicted?



    This data was developed using ab75879, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
    Immunocytochemistry - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
    This data was developed using ab75879, the same antibody clone in a different buffer formulation.Immunofluorescence staining of HeLa cells with purified ab75879 at a working dilution of 1/50, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab75879 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
  • Immunocytochemistry - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
    Immunocytochemistry - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
    This data was developed using ab75879, the same antibody clone in a different buffer formulation.Unpurified ab75879, at a 1/100 dilution, staining Epsin 1 in SH-SY5Y cells by Immunofluorescence.
  • Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
    Western blot - Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
    All lanes : Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/2000 dilution (unpurified)

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit-HRP at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 58 kDa



    This data was developed using ab75879, the same antibody clone in a different buffer formulation.

     

  • Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)
    Anti-Epsin 1 antibody [EPR3023] - BSA and Azide free (ab247415)

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