Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GITR antibody [CAL8] - BSA and Azide free (ab251601)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CAL8] to GITR - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-GITR antibody [CAL8] - BSA and Azide free
    See all GITR primary antibodies

  • Description

    Rabbit monoclonal [CAL8] to GITR - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil tissue. Flow Cyt: Human PBMCs.

  • General notes

    ab251601 is the carrier-free version of ab237714 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251601 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labeled as TNFRSF18.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL8] - BSA and Azide free (ab251601)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GITR antibody [CAL8] - BSA and Azide free (ab251601)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling GITR with ab237714 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the human tonsil tissue is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237714).

  • Flow Cytometry - Anti-GITR antibody [CAL8] - BSA and Azide free (ab251601)
    Flow Cytometry - Anti-GITR antibody [CAL8] - BSA and Azide free (ab251601)

    Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized human peripheral blood mononuclear cell (PBMC) treated with 10μg/ml PHA for 48hrs, labeling GITR with ab237714 at 1/500 (right) compared with a rabbit IgG (left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/5000 dilution was used as the secondary antibody. Cells were surface stained with anti-CD25 conjugated to BV421. Then fixed with 2% PFA followed by intracellular staining rabbit IgG (Left) or ab237714 (Right).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237714).

  • Anti-GITR antibody [CAL8] - BSA and Azide free (ab251601)
    Anti-GITR antibody [CAL8] - BSA and Azide free (ab251601)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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