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Anti-Calnexin antibody (ab265604)

Anti-Calnexin antibody (ab265604)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Calnexin
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Calnexin antibody
    See all Calnexin primary antibodies
  • Description

    Rabbit polyclonal to Calnexin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog, Orangutan
  • Immunogen

    Synthetic peptide within Human Calnexin aa 542-592. The exact sequence is proprietary.
    Database link: P27824

  • Positive control

    • IHC-P: Human breast carcinoma tissue. IP: HeLa whole cell lysate. WB: HeLa, HEK-293T, NIH/3T3 and Jurkat whole cell lysate.
  • General notes

     

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • ER
    • Neuroscience
    • Sensory System
    • Visual system

Images

  • Western blot - Anti-Calnexin antibody (ab265604)
    Western blot - Anti-Calnexin antibody (ab265604)
    All lanes : Anti-Calnexin antibody (ab265604) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HEK-293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 68 kDa


    Exposure time: 10 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody (ab265604)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody (ab265604)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling Calnexin with ab265604 at 1/1000 dilution. Detection: DAB.

  • Immunoprecipitation - Anti-Calnexin antibody (ab265604)
    Immunoprecipitation - Anti-Calnexin antibody (ab265604)

    Calnexin was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab265604 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab265604 at 1 µg/ml.

    Lane 1: ab265604 IP in HeLa whole cell lysate.
    Lane 2: Control IgG.

    Exposure time: 3 secs.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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