Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Calnexin antibody [EPR21205] (ab213243)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21205] to Calnexin
  • Suitable for: ICC/IF, WB
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Calnexin antibody [EPR21205]
    See all Calnexin primary antibodies

  • Description

    Rabbit monoclonal [EPR21205] to Calnexin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein (His-tag) corresponding to Mouse Calnexin. Expressed in HEK293 Cells
    Database link: NP_031623.1

  • Positive control

    • WB: NIH3T3, HeLa, HAP1 ICC-IF: HeLa and NIH3T3 cells.

  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21205

  • Isotype

    IgG1

  • Research areas

Images

  • Western blot - Anti-Calnexin antibody [EPR21205] (ab213243)
    Western blot - Anti-Calnexin antibody [EPR21205] (ab213243)

    Lane 1: NIH3T3 whole cell lysate (10 µg)
    Lane 2: Mouse brain whole tissue lysate (10 µg)
    Lane 3: HeLa whole cell lysate (10 µg)
    Lane 4: Hap1 Wild-type whole cell lysate (10 µg)
    Lane 5: CANX knockout Hap1 whole cell lysate (10 µg)

    Lanes 1 - 5: Merged signal (red and green). Green - ab213243 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab213243 was shown to specifically react with Calnexin in wild-type HAP1 cells as signal was lost in Calnexin (CANX) knockout cells. Wild-type and CANX knockout samples were subjected to SDS-PAGE. Nitrocellulose membranes were blocked in 3% milk in TBST before ab213243 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1µg/mL and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody [EPR21205] (ab213243)
    Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody [EPR21205] (ab213243)

    ab213243 staining Calnexin in NIH3T3 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab213243 at 0.05ugml then detected with an Alexa Fluor® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red).

     

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody [EPR21205] (ab213243)
    Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody [EPR21205] (ab213243)

    ab213243 staining Calnexin in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab213243 at 0.05ugml then detected with an Alexa Fluor® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red).

     

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Anti-Calnexin antibody [EPR21205] (ab213243)
    Anti-Calnexin antibody [EPR21205] (ab213243)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Calnexin antibody [EPR21205] (ab213243)

© 2021 Astana Biomed Group LLP. All rights reserved.