Anti-Calnexin (phospho S583) antibody (ab58503)
Key features and details
- Rabbit polyclonal to Calnexin (phospho S583)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Calnexin (phospho S583) antibody
See all Calnexin primary antibodies -
Description
Rabbit polyclonal to Calnexin (phospho S583) -
Host species
Rabbit -
Specificity
ab58503 detects endogenous levels of Calnexin - ER membrane marker only when phosphorylated at serine 583. Recognizes ER membrane, mitochondria and cis-Golgi -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phosphopeptide derived from human Calnexin - ER membrane marker around the phosphorylation site of serine 583 (N-R-SP-P-R).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab58503 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Calnexin (phospho S583) antibody (ab58503)All lanes : Anti-Calnexin (phospho S583) antibody (ab58503) at 1/500 dilution
Lane 1 : HeLa cell extract treated with EGF (200ng/ml, 30mins)
Lane 2 : HeLa cell extract treated with EGF (200ng/ml, 30mins) with immunising peptide
Predicted band size: 90 kDa
Observed band size: 90 kDa
