Anti-DEPDC5 antibody [EPR20497-23] (ab213181)
![Anti-DEPDC5 antibody [EPR20497-23] (ab213181)](https://www.abcam.com/ps/products/213/ab213181/Images/ab213181-388953-anti-depdc5-antibody-epr2049723-western-blot-wildtype-mouse-e14-brain-depdc5-knockout-mouse-e14-brain-lysates.jpg "Anti-DEPDC5 antibody [EPR20497-23] (ab213181)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20497-23] to DEPDC5
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse
Overview
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Product name
Anti-DEPDC5 antibody [EPR20497-23]
See all DEPDC5 primary antibodies -
Description
Rabbit monoclonal [EPR20497-23] to DEPDC5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type mouse E14 brain lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20497-23 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DEPDC5 antibody [EPR20497-23] (ab213181)All lanes : Anti-DEPDC5 antibody [EPR20497-23] (ab213181) at 1/1000 dilution
Lane 1 : Wild-type mouse E14 brain tissue lysate
Lane 2 : DEPDC5 knockout mouse E14 brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 181 kDa
Observed band size: 181 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The wild-type and DEPDC5 knockout mouse E14 brain tissue lysates were kindly provided by an anonymous collaborator.
ab213181 was shown to specifically react with DEPDC5 in wild-type mouse E14 brain tissue as signal was lost in DEPDC5 knockout tissue. Wild-type and DEPDC5 knockout samples were subjected to SDS-PAGE. ab213181 and ab129002 (Rabbit anti-Vinculin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/5000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100, 000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
Exposure time: 26 seconds.
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![Anti-DEPDC5 antibody [EPR20497-23] (ab213181)](https://www.abcam.com/ps/products/213/ab213181/Images/ab213181-2-benefits-of-recombinant-antibodies.png)
Anti-DEPDC5 antibody [EPR20497-23] (ab213181)