Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)
![Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)](https://www.abcam.com/ps/products/154/ab154791/Images/ab154791-161283-anti-ilf2-antibody-epr10695b-western-blot.jpg "Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10695(B)] to ILF2/NF45
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-ILF2/NF45 antibody [EPR10695(B)]
See all ILF2/NF45 primary antibodies -
Description
Rabbit monoclonal [EPR10695(B)] to ILF2/NF45 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human ILF2/NF45.
Database link: Q12905 -
Positive control
- Purchase matching WB positive control:Recombinant Human ILF2/NF45 protein
- K562, Raji, A431 and HeLa cell lysates; Human spleen tissue; Human thyroid gland carcinoma tissue.
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General notes
This product was previously labelled as ILF2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10695(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)All lanes : Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 43 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)](https://www.abcam.com/ps/products/154/ab154791/Images/ab154791-161282-anti-ilf2-antibody-epr10695b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling ILF2/NF45 with ab154791 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)](https://www.abcam.com/ps/products/154/ab154791/Images/ab154791-161281-anti-ilf2-antibody-epr10695b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling ILF2/NF45 with ab154791 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)](https://www.abcam.com/ps/products/154/ab154791/Images/ab154791-161280-anti-ilf2-antibody-epr10695b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)Immunohistochemical analysis of paraffin embedded Human glioma tissue using ab154791 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)](https://www.abcam.com/ps/products/154/ab154791/Images/ab154791-161279-anti-ilf2-antibody-epr10695b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab154791 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)](https://www.abcam.com/ps/products/154/ab154791/Images/ab154791-161278-anti-ilf2-antibody-epr10695b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154791 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)](https://www.abcam.com/ps/products/154/ab154791/Images/ab154791-161277-anti-ilf2-antibody-epr10695b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab154791 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)](https://www.abcam.com/ps/products/154/ab154791/Images/ab154791-215932-anti-ilf2-antibody-epr10695b-flow-cytometry.jpg)
Flow Cytometry - Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)Overlay histogram showing HeLa cells stained with ab154791 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab154791, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)](https://www.abcam.com/ps/products/154/ab154791/Images/ab154791-9-benefits-of-recombinant-antibodies.png)
Anti-ILF2/NF45 antibody [EPR10695(B)] (ab154791)
