Anti-ILF2/NF45 antibody (ab93838)
Key features and details
- Rabbit polyclonal to ILF2/NF45
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ILF2/NF45 antibody
See all ILF2/NF45 primary antibodies -
Description
Rabbit polyclonal to ILF2/NF45 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human ILF2/NF45 aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab104699) -
Positive control
- Purchase matching WB positive control:Recombinant Human ILF2/NF45 protein
- This antibody gave a positive signal in both HeLa and Jurkat whole cell lysates.
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General notes
This product was previously labelled as ILF2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatCowDogOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).Target
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Function
Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. -
Sequence similarities
Contains 1 DZF domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 539760 Cow
- Entrez Gene: 3608 Human
- Entrez Gene: 67781 Mouse
- Entrez Gene: 310612 Rat
- Omim: 603181 Human
- SwissProt: Q12905 Human
- SwissProt: Q9CXY6 Mouse
- SwissProt: Q5RFJ1 Orangutan
see all -
Alternative names
- HGNC:6037 antibody
- ILF 2 antibody
- ilf2 antibody
see all
Images
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Western blot - Anti-ILF2/NF45 antibody (ab93838)All lanes : Anti-ILF2/NF45 antibody (ab93838) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Additional bands at: 100 kDa, 22 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Datasheets and documents
References (0)
ab93838 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-ILF2/NF45 antibody (ab93838)All lanes : Anti-ILF2/NF45 antibody (ab93838) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Additional bands at: 100 kDa, 22 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
