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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-ILF2/NF45 antibody (ab93838)

Price and availability

268 032 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-ILF2/NF45 antibody (ab93838)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to ILF2/NF45
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ILF2/NF45 antibody
    See all ILF2/NF45 primary antibodies
  • Description

    Rabbit polyclonal to ILF2/NF45
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human ILF2/NF45 aa 200-300 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab104699)

  • Positive control

    • Purchase matching WB positive control:Recombinant Human ILF2/NF45 protein
    • This antibody gave a positive signal in both HeLa and Jurkat whole cell lysates.
  • General notes

     This product was previously labelled as ILF2

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human ILF2/NF45 protein (ab132371)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab93838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Rat
Cow
Dog
Orangutan
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

  • Function

    Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.
  • Sequence similarities

    Contains 1 DZF domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession Q12905 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 539760 Cow
    • Entrez Gene: 3608 Human
    • Entrez Gene: 67781 Mouse
    • Entrez Gene: 310612 Rat
    • Omim: 603181 Human
    • SwissProt: Q12905 Human
    • SwissProt: Q9CXY6 Mouse
    • SwissProt: Q5RFJ1 Orangutan
    • SwissProt: Q7TP98 Rat
    • Unigene: 75117 Human
    • Unigene: 227258 Mouse
    • Unigene: 137428 Rat
    see all
  • Alternative names

    • HGNC:6037 antibody
    • ILF 2 antibody
    • ilf2 antibody
    • ILF2_HUMAN antibody
    • Interleukin enhancer binding factor 2 antibody
    • Interleukin enhancer binding factor 2, 45kDa antibody
    • Interleukin enhancer-binding factor 2 antibody
    • MGC8391 antibody
    • NF 45 antibody
    • NF45 antibody
    • Nuclear factor of activated T cells 45 kDa antibody
    • Nuclear factor of activated T-cells 45 kDa antibody
    • PRO3063 antibody
    see all

Images

  • Western blot - Anti-ILF2/NF45 antibody (ab93838)
    Western blot - Anti-ILF2/NF45 antibody (ab93838)
    All lanes : Anti-ILF2/NF45 antibody (ab93838) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa
    Additional bands at: 100 kDa, 22 kDa, 65 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab93838? Please let us know so that we can cite the reference in this datasheet.

    ab93838 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-ILF2/NF45 antibody (ab93838)
      Western blot - Anti-ILF2/NF45 antibody (ab93838)
      All lanes : Anti-ILF2/NF45 antibody (ab93838) at 1 µg/ml

      Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 43 kDa
      Observed band size: 43 kDa
      Additional bands at: 100 kDa, 22 kDa, 65 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 4 minutes

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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