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Anti-beta Amyloid 1-42 antibody [mOC98] - Conformation-Specific (ab201061)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [mOC98] to beta Amyloid 1-42 - Conformation-Specific
  • Suitable for: IHC-P, Dot blot
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-beta Amyloid 1-42 antibody [mOC98] - Conformation-Specific
    See all beta Amyloid 1-42 primary antibodies

  • Description

    Rabbit monoclonal [mOC98] to beta Amyloid 1-42 - Conformation-Specific

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Dot
    Mouse
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Other Immunogen Type corresponding to Human beta Amyloid 1-42. Amyloid beta 1-42 fibrils were used as the immunogen.
    Database link: P05067

  • Positive control

    • beta Amyloid (Aß) 1-42 IHC-P: FFPE human Alzheimer hippocampus tissue sections.

  • General notes

    This antibody was developed as part of a collaboration between Abcam and Professor Charles Glabe, UC Irvine.

    ab201061 recognizes a conformation-specific, discontinuous epitope of beta Amyloid that maps to segments AEFRHD and EDVGSNK (Hatami et al. 2014).

    For further information on the immunogen, please refer to Hatami et al. 2014 and Kayed et al. 2007.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    mOC98

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-42 antibody [mOC98] - Conformation-Specific (ab201061)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-42 antibody [mOC98] - Conformation-Specific (ab201061)

    IHC image of beta Amyloid 1-42 staining in human Alzheimer hippocampus formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab201062, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Dot Blot - Anti-beta Amyloid 1-42 antibody [mOC98] - Conformation-Specific (ab201061)
    Dot Blot - Anti-beta Amyloid 1-42 antibody [mOC98] - Conformation-Specific (ab201061)

    Dot blot analysis of beta Amyloid 1-42 labeled with ab201061 at 1/10000 dilution.
    Lane 1: beta Amyloid (Aβ) 1-40.
    Lane 2: beta Amyloid (Aβ) 1-42.
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/5000 dilution was used as secondary antibody.
    Blocking and diluting buffer: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    Antibody reactivity was assessed using a dot blot, which is a non-quantitative method that maintains the native conformation of beta Amyloid. Beta Amyloid 1-40 and 1-42 peptides underwent the following aggregation conditions before being spotted onto a nitrocellulose membrane and detected using ab201061:
    Monomers: 0.3 mg of beta Amyloid peptide was dissolved in 30 µl 100 mM NaOH and incubated at room temperature for 10 minutes. It was then diluted with 970 µl of 1% SDS and boiled for five minutes.
    Oligomers: 0.3 mg of beta Amyloid peptide was dissolved in 30 µl 100 mM NaOH and incubated at room temperature for 10 minutes. It was then diluted with 970 µl of 10 mM phosphate buffer pH 7.4 containing 0.02% sodium azide and incubated at room temperature for four days.
    Fibrils: 0.3 mg of beta Amyloid peptide was dissolved in 1 ml 50% hexafluoroisopropanol (HFIP) with 0.02% sodium azide. It was then stirred constantly for nine days; the first seven with a cap on and the final two with the cap removed to allow evaporation of the HFIP. Fibrils were then sedimented at 20,000 rpm in a microcentrifuge for 20 minutes and resuspended in 1 ml of PBS + 0.02% sodium azide.

  • Dot Blot - Anti-beta Amyloid 1-42 antibody [mOC98] - Conformation-Specific (ab201061)
    Dot Blot - Anti-beta Amyloid 1-42 antibody [mOC98] - Conformation-Specific (ab201061)

    Negative control (secondary ab only):

    Lane 1: beta Amyloid (Aβ) 1-40.
    Lane 2: beta Amyloid (Aβ) 1-42.

    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/5000 dilution was used as secondary antibody.
    Blocking and diluting buffer: 5% NFDM/TBST.
    Exposure time: 30 seconds.

  • Anti-beta Amyloid 1-42 antibody [mOC98] - Conformation-Specific (ab201061)
    Anti-beta Amyloid 1-42 antibody [mOC98] - Conformation-Specific (ab201061)

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