Anti-CREBBP antibody [CBP5I001] (ab50702)
![Anti-CREBBP antibody [CBP5I001] (ab50702)](https://www.abcam.com/ps/products/50/ab50702/Images/ab50702-278184-anti-kat3a-cbp-antibody-cbp5i001-western-blot.jpg "Anti-CREBBP antibody [CBP5I001] (ab50702)")
Key features and details
- Mouse monoclonal [CBP5I001] to CREBBP
- Suitable for: IHC-P, WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-CREBBP antibody [CBP5I001]
See all CREBBP primary antibodies -
Description
Mouse monoclonal [CBP5I001] to CREBBP -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB MouseHuman
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Immunogen
Recombinant full length protein corresponding to Human CREBBP.
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Positive control
- HeLa whole cell lysate (ab150035); NIH3T3 whole cell lysate. IHC-P: Human pancreas FFPE tissue sections.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted(approximately 95%) fetal bovine serum, and filtered through a 0.22 µm membrane. -
Clonality
Monoclonal -
Clone number
CBP5I001 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-CREBBP antibody [CBP5I001] (ab50702)
Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
Lane 2: CREBBP knockout HAP1 whole cell lysate (40 µg)
Lane 3: HeLa whole cell lysate (40 µg)
Lane 4: NIH3T3 whole cell lysate (40 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab50702 observed at 265 kDa. Red - loading control, ab176560, observed at 50 kDa.
ab50702 was shown to specifically recognize CREBBP in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when CREBBP knockout samples were examined. Wild-type and CREBBP knockout samples were subjected to SDS-PAGE. Ab50702 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/100 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody [CBP5I001] (ab50702)](https://www.abcam.com/ps/products/50/ab50702/Images/ab50702-172906-anti-kat3a-cbp-antibody-cbp5i001-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody [CBP5I001] (ab50702)IHC image of CREBBP staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50702, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Immunocytochemistry/ Immunofluorescence - Anti-CREBBP antibody [CBP5I001] (ab50702)](https://www.abcam.com/ps/products/50/ab50702/Images/ab50702-62379-anti-kat3a-cbp-antibody-cbp5i001-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CREBBP antibody [CBP5I001] (ab50702)Staining of HeLa cell lysate with ab50702 at a dilution of 1/5 and Goat anti-mouse secondary at 1/200. -
![Western blot - Anti-CREBBP antibody [CBP5I001] (ab50702)](https://www.abcam.com/ps/products/50/ab50702/Images/ab50702-62382-anti-kat3a-cbp-antibody-cbp5i001-western-blot.jpg)
Western blot - Anti-CREBBP antibody [CBP5I001] (ab50702)Anti-CREBBP antibody [CBP5I001] (ab50702) at 1/30 dilution + RIPA lysate of HeLa cells at 500 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 265 kDa
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![Western blot - Anti-CREBBP antibody [CBP5I001] (ab50702)](https://www.abcam.com/ps/products/50/ab50702/Images/ab50702-62381-anti-kat3a-cbp-antibody-cbp5i001-western-blot.jpg)
Western blot - Anti-CREBBP antibody [CBP5I001] (ab50702)Anti-CREBBP antibody [CBP5I001] (ab50702) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 265 kDa
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![Western blot - Anti-CREBBP antibody [CBP5I001] (ab50702)](https://www.abcam.com/ps/products/50/ab50702/Images/ab50702-62380-anti-kat3a-cbp-antibody-cbp5i001-western-blot.jpg)
Western blot - Anti-CREBBP antibody [CBP5I001] (ab50702)Anti-CREBBP antibody [CBP5I001] (ab50702) at 1/100 dilution + HeLa whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 265 kDa
