Anti-CREBBP (acetyl K1535) antibody (ab61242)
Key features and details
- Rabbit polyclonal to CREBBP (acetyl K1535)
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CREBBP (acetyl K1535) antibody
See all CREBBP primary antibodies -
Description
Rabbit polyclonal to CREBBP (acetyl K1535) -
Host species
Rabbit -
Specificity
Detects endogenous levels of CBP only when acetylated at lysine 1535. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
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Immunogen
Synthetic peptide corresponding to Human CREBBP (acetyl K1535).
Sequence:SAKAcEL
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Positive control
- Human lung carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific acetylated peptide. The antibody against non acetylated peptide was removed by chromatography using non acetylated peptide corresponding to the acetylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP (acetyl K1535) antibody (ab61242)
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using CREBBP (acetyl K1535) antibody (ab61242) at 1/50 dilution, in the presence (right panel) or absence (left panel) of acetylated peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-CREBBP (acetyl K1535) antibody (ab61242)ICC/IF image of ab61242 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61242, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
