Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GSTA1 antibody [EPR19969] (ab207413)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19969] to GSTA1
  • Suitable for: WB, IP
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-GSTA1 antibody [EPR19969]
    See all GSTA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19969] to GSTA1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human GSTA1 full length recombinant protein; HepG2 and Caco-2 whole cell lysates; Human pancreas, fetal lung, fetal liver, testis and fetal kidney lysates. IP: HepG2 whole cell lysate.

  • General notes

    Previously labelled as Glutathione S Transferase alpha 1. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-GSTA1 antibody [EPR19969] (ab207413)
    Western blot - Anti-GSTA1 antibody [EPR19969] (ab207413)
    All lanes : Anti-GSTA1 antibody [EPR19969] (ab207413) at 1/2000 dilution

    Lane 1 : Human GSTA1 full length recombinant protein
    Lane 2 : Human GSTA2 full length recombinant protein
    Lane 3 : Human GSTA3 full length recombinant protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    GSTA1, A2, and A3 are His-Tagged full-length (aa1-222) recombinant proteins.

    GSTA4 and A5 were not recognized by ab207413 (data not shown).

  • Immunoprecipitation - Anti-GSTA1 antibody [EPR19969] (ab207413)
    Immunoprecipitation - Anti-GSTA1 antibody [EPR19969] (ab207413)

    GSTA1 was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab207413 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab207413 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HepG2 whole cell lysate, 10µg (Input).

    Lane 2: ab207413 IP in HepG2 whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab207413 in HepG2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Western blot - Anti-GSTA1 antibody [EPR19969] (ab207413)
    Western blot - Anti-GSTA1 antibody [EPR19969] (ab207413)
    All lanes : Anti-GSTA1 antibody [EPR19969] (ab207413) at 1/5000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 3 : Human pancreas lysate
    Lane 4 : Human fetal lung lysate
    Lane 5 : Human fetal liver lysate
    Lane 6 : Human testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 3-6 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1,2,3 and 4: 3 minutes; Lane 5 and 6: 1 second.

  • Western blot - Anti-GSTA1 antibody [EPR19969] (ab207413)
    Western blot - Anti-GSTA1 antibody [EPR19969] (ab207413)
    Anti-GSTA1 antibody [EPR19969] (ab207413) at 1/2000 dilution + Human fetal kidney lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-GSTA1 antibody [EPR19969] (ab207413)
    Anti-GSTA1 antibody [EPR19969] (ab207413)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-GSTA1 antibody [EPR19969] (ab207413)

© 2021 Astana Biomed Group LLP. All rights reserved.